Immunohistochemical study of glutathione S-transferase (GST-.PI.) on hepatocellular carcinoma.
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چکیده
منابع مشابه
Glutathione-S-transferase-pi (GST-pi) expression in renal cell carcinoma
Multidrug resistance correlates with unfavourable treatment outcomes in numerous cancers including renal cell carcinoma. The expression and clinical relevance of Glutathione-S-transferase-pi (GST-pi), a multidrug resistance factor, in kidney tumors remain controversial. We analyzed the expression of GST-pi in 60 formalin-fixed, paraffin-embedded renal cell carcinoma samples by immunohistochemis...
متن کاملGlutathione Transferase (GST)-Activated Prodrugs
Glutathione transferase (formerly GST) catalyzes the inactivation of various electrophile-producing anticancer agents via conjugation to the tripeptide glutathione. Moreover, several data link the overexpression of some GSTs, in particular GSTP1-1, to both natural and acquired resistance to various structurally unrelated anticancer drugs. Tumor overexpression of these proteins has provided a ra...
متن کاملMolecular cloning and expression study of pi-class glutathione S-transferase (pi-GST) and selenium-dependent glutathione peroxidase (Se-GPx) transcripts in the freshwater bivalve Dreissena polymorpha.
Glutathione S-transferases (GST) and glutathione peroxidases (GPx) are essential components of cellular detoxification systems. We identified GST and GPx transcripts in the freshwater bivalve Dreissena polymorpha, their full-length coding sequences were obtained by reverse-transcription PCR using degenerated primers followed by 5' and 3' RACE-PCR (rapid amplification of cDNA ends-PCR). The cDNA...
متن کاملStudy on Binding Affinity of a Glutathione S-Transferase (GST) Fusion Protein to DNA Probe
The aims of this work were to: i) purify GST-fusion protein from bacterial cell extracts of Escherichia coli; ii) quantify the protein by SDS PAGE and Bradford assay; iii) determine protein-DNA interaction of the purified protein by Electrophoretic Mobility Shift Assay. Bacterial culture prepared by inoculation of a single E. coli colony that had a GST fusion protein (gst: six-X10 hd) construct...
متن کاملImmunohistochemical expression of pi-class glutathione S-transferase is down-regulated in adenocarcinoma of the prostate.
BACKGROUND Glutathione S-transferase is often up-regulated in neoplastic tissues. A single previous study found a loss of expression associated with carcinogenesis of the prostate. METHODS To extend these results, the authors performed immunohistochemical staining for the pi-class of glutathione S-transferase (GSTpi) on 74 archival sequential prostate specimens. The antibody used was derived ...
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ژورنال
عنوان ژورنال: Kanzo
سال: 1986
ISSN: 0451-4203,1881-3593
DOI: 10.2957/kanzo.27.1439